作者: |
Niu Lingchuan, Zhou Jia, Huang Ying, Chen Yan, Li Changqing
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刊名: |
Neurology India, 2010, Vol.58 (2), pp.195 |
来源数据库: |
Directory of Open Access Journals |
关键词: |
Axon regeneration; cerebral ischemia; dual-acid cyclic adenosine monophosphate; growth associated Protein-43; RhoA.; |
原始语种摘要: |
Background: There is no effective axon regeneration in adult mammalians. Objective: To investigate the effects of dual-acid cyclic adenosine monophosphate (db-cAMP) on the axon regeneration, motor function recovery and RhoA signal pathway in cerebral ischemia-reperfusion rats, and to explore the possible clinical application and mechanism. Materials and Methods: Middle cerebral artery ischemia-reperfusion model was established by nylon monofilament occlusion method in 105 Sprague-Dawley (SD) rats. Semi-quantitative Western blot analysis was used to assess protein expression level of growth-associated protein-43 (GAP-43) and RhoA. Montoya staircase test score was used to test the motor function of affected forelimb. Results: Compared to the ischemia group, the staircase test score in the... db-cAMP group was increased significantly at 30-day (P < 0.05), and GAP-43 protein expression in the db-cAMP group was enhanced significantly at 7-day and 14-day (P < 0.05), and RhoA protein expression in the db-cAMP group was decreased significantly between 24 h to 14-day (P < 0.01). Conclusion: These results show that db-cAMP can promote axon regeneration and the recovery of motor function by inhibiting RhoA signal pathway.
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