Screening of Colletotrichum isolates from the Amazon biodiversity as producers of laccases using a simple methodology / Seleção de isolados de Colletotrichum da biodiversidade da Amazônia como produtores de lacases utilizando uma metodologia simplificada
作者: Gilmara Carvalho MartinezEllen Cristine GieseJosé Odair PereiraRobert F. H. DekkerAneli de Melo Barbosa
刊名: Semina : Ciências Agrárias, 2009, Vol.30 (2), pp.397
来源数据库: Directory of Open Access Journals
关键词: ColletotrichumLacasesBiodiversidade amazônica.
原始语种摘要: This work presents a simple and rapid technique to select fungal strains with the ability to produce laccases. Fifteen strains of Colletotrichum spp. from the Amazon region (isolated from cumarú, puerária, cupuaçu and weed plants), as well as a strain isolated from “espinheira santa” in the south of Brazil, were found to produce laccases. Laccase activity was assayed using ABTS and DMP as putative substrates. Only Colletotrichum isolates of puero (P2 and P3) did not present activity towards DMP. Isolates D3 (weed plants), M3 (espinheira santa), C2 and C3 (cumarú) were selected, and were compared with the basidiomycete Pleurotus ostreatus used as a reference laccase producer under stationary and submerged culture conditions. Highest laccase activity was detected only in the stationary...
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关键词翻译
关键词翻译
  • isolates 单离体
  • DMP Dump Management Program
  • activity 活度
  • basidiomycete 担子菌
  • biodiversity 生物多样性
  • stationary 固定的
  • methodology 方法学
  • submerged 暗礁
  • laccase 漆酶
  • region 地域