Development and validation of HPTLC method for the estimation of clotrimazole in bulk drug and tablet formulation
作者: Parmar ParulMehta Ankita
刊名: Indian Journal of Pharmaceutical Sciences, 2009, Vol.71 (4), pp.451
来源数据库: Directory of Open Access Journals
关键词: HPTLC method of estimationClotrimazoleTablets
原始语种摘要: A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the determination of clotrimazole in bulk drug and tablet dosage form. The stationary phase used was precoated silica gel 60F 254 . The mobile phase used was a mixture of cyclohexane:toluene:methanol:triethyleamine (8:2:0.5:0.2 v/v/v/v). The detection of spot was carried out at 262 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 1000 ng/spot for clotrimazole. The limit of detection and the limit of quantification for clotrimazole were found to be 50 ng/spot and 200 ng/spot, respectively. The proposed method can be successfully used to determine the drug...
全文获取路径: DOAJ  (合作)
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影响因子:0.338 (2012)

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关键词翻译
关键词翻译
  • clotrimazole 克霉唑
  • tablet 药片
  • formulation 配方
  • determination 测定
  • calibration 校准
  • estimation 估计
  • HPTLC 高效薄层色谱法
  • validation 证实
  • successfully 成功地
  • limit 界限