Towards an intraoperative engineering of osteogenic and vasculogenic grafts from the stromal vascular fraction of human adipose tissue
作者: AM MüllerA MehrkensDJ SchäferC JaquieryS GüvenM LehmickeR MartinettiI FarhadiM JakobA ScherberichI Martin
刊名: European Cells and Materials (ECM), 2010, Vol.19 , pp.127
来源数据库: Directory of Open Access Journals
关键词: intraoperativeosteogenicvasculogenictissue engineered graftshumanadipose-derived cellsceramic materials
原始语种摘要: Grafts generated by cultivation of progenitor cells from the stromal vascular fraction of human adipose tissue have been proven to have osteogenic and vasculogenic properties in vivo. However, in vitro manufacture of such implants is challenged by complex, impractical and expensive processes, and requires implantation in a separate surgery. This study investigates the feasibility of an intraoperative approach to engineer cell-based bone grafts with tissue harvest, cell isolation, cell seeding onto a scaffold and subsequent implantation within a few hours. Freshly isolated adipose tissue cells from a total of 11 donors, containing variable fractions of mesenchymal and endothelial progenitors, were embedded at different densities in a fibrin hydrogel, which was wrapped around bone...
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影响因子:4.558 (2012)

  • osteogenic 成骨的
  • adipose 脂肪的
  • vascular 维管的
  • stromal 基质的
  • tissue 组织
  • tricalcium 三钙
  • mesenchymal 间叶细胞的
  • hydrogel 水凝胶
  • fraction 分数
  • constructs 建筑图纸生成系统