Fasudil inhibits actin polymerization and collagen synthesis and induces apoptosis in human urethral scar fibroblasts via the Rho/ROCK pathway
作者: Xiao-Dong LiYu-Peng WuShao-Hao ChenYing-Chun LiangTing-Ting LinTian LinYong WeiXue-Yi XueQing-Shui ZhengNing Xu
作者单位: 1Department of Urology
刊名: Drug Design, Development and Therapy, 2018, Vol.2018 (default), pp.2707-2713
来源数据库: Dove Journal
DOI: 10.2147/DDDT.S156095
原始语种摘要: Purpose: To examine the effects and mechanism of action of fasudil on cytoskeletal polymerization, collagen synthesis and apoptosis in fibroblasts derived from human urethral scar tissue.Materials and Methods: Fibroblasts treated with or without transforming growth factor β1 (TGF-β1, 10 ng/mL) were incubated with fasudil (12.5, 25, 50 μmol/L) for 24 h. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were used to determine the expression of Arp2, Arp3, WASP and WAVE2. Collagen I and III protein levels were also evaluated by western blotting. The filamentous actin cytoskeleton was examined by immunofluorescence and epifluorescence microscopy. An Annexin V-FITC/Propidium Iodide staining assay was used to investigate apoptosis.Results: TGF-β1-dependent...
全文获取路径: Dove 
影响因子:3.486 (2012)

  • urethral 尿道的
  • collagen 胶骨硬蛋白质
  • pathway 轨道
  • apoptosis 细胞死
  • actin 肌动蛋白
  • human 人的
  • blotting 浸透
  • synthesis 合成
  • polymerization 聚合酌
  • Collagen 胶原质