Objective: to identify prognostic significance of microRNA-100 in solid tumor.Methods: literature search was conducted in PubMed, Embase, Web of Science, using the following words “(microRNA-100 or miR-100 or mir100) and (tumor or neoplasm or cancer or carcinoma or malignancy)”. The search was updated by July 10th 2016. Newcastle–Ottawa scale was performed to evaluate the quality of studies. Pooled hazard ratio with 95% confidence interval for patients’ survival was calculated by using a fixed-effects or random-effects model according to heterogeneity. Subgroup analysis, sensitive analysis, and meta-regression were used to investigate sources of heterogeneity. Publication bias was evaluated by Begg’s test and Egger’s test.Results: a total of 16 articles with 1501 patients were included in... the present meta-analysis. It was demonstrated that lower expression of miR-100 played a negative role in overall survival of patients with solid tumor (hazard ratio 1.92, 95% confidence interval 1.25–2.94). In addition, the association between miR-100 and prognosis was also revealed in the following subgroups: non-small cell lung cancer (hazard ratio 2.46, 95% confidence interval 1.98-3.06), epithelial ovarian cancer (hazard ratio 2.29, 95% confidence interval 1.72-3.04) and bladder cancer (hazard ratio 4.14, 95% confidence interval 1.85-9.27).Conclusion: this meta-analysis indicate that lower expression of miR-100 is related to poorer overall survival in solid tumor, especially for patients with non-small cell lung cancer, epithelial ovarian cancer and bladder cancer. MiR-100 is a promising prognosis predictor and may be a potential target for therapy in the future.