The study was carried out to investigate the distribution and prevalence of some multidrug resistant nosocomial pathogens in various selected hospitals in Ilorin. Various hospitals sections were assessed. This finding revealed that Klebsiella pneumoniae was predominant with 14.9% followed by E. coli, Streptococcus sp and Acinetobacter sp of 12.4%, 12.3% and 12.2% respectively. All Gram negative bacteria were susceptible to Ofloxacin, Ciprofloxacin Cephalexin and resistant to Chloramphenicol, Septrin, Vancomycin and Ampicillin while, Gram positive bacteria were found to be susceptible to Ofloxacin, Vancomycin and Ampicillin and resistant to septrin, Augmentin and tetracycline. Acinetobacter and Pseudomonas were found to display high level of resistance to most tested antibiotics with... varying magnitude. Six of the isolates harboured R-plasmid acquired transfer of mobile genetic element. Gene coding for antibiotics resistance were located on the plasmid while the other three isolates without plasmids may have their gene coding located on their chromosomal DNA. It was concluded that the principle of antimicrobial stewardship is urgently needed to preserve efficacy of available antimicrobial agents.