The CDK Pef1 and protein phosphatase 4 oppose each other for regulating cohesin binding to fission yeast chromosomes
作者: Adrien BirotMarta Tormos-PérezSabine VaurAmélie FeytoutJulien JaegyDácil Alonso GilStéphanie VazquezKarl EkwallJean-Paul Javerzat
作者单位: 11 Institut de Biochimie et Génétique Cellulaires, UMR 5095 CNRS - Université de Bordeaux Bordeaux France
22 Department of Biosciences and Nutrition Karolinska Institutet Huddinge Sweden
刊名: eLife, 2020, Vol.9
来源数据库: eLife Sciences Publications, Ltd
DOI: 10.7554/eLife.50556
关键词: CohesinPhosphataseCDKCohesionLoop extrusionS. pombe
原始语种摘要: Cohesin has essential roles in chromosome structure, segregation and repair. Cohesin binding to chromosomes is catalyzed by the cohesin loader, Mis4 in fission yeast. How cells fine tune cohesin deposition is largely unknown. Here, we provide evidence that Mis4 activity is regulated by phosphorylation of its cohesin substrate. A genetic screen for negative regulators of Mis4 yielded a CDK called Pef1, whose closest human homologue is CDK5. Inhibition of Pef1 kinase activity rescued cohesin loader deficiencies. In an otherwise wild-type background, Pef1 ablation stimulated cohesin binding to its regular sites along chromosomes while ablating Protein Phosphatase 4 had the opposite effect. Pef1 and PP4 control the phosphorylation state of the cohesin kleisin Rad21. The CDK phosphorylates...
全文获取路径: eLife科学出版社 

  • regulating 蝶的
  • yeast 酵母
  • segregation 分凝
  • binding 装订
  • screen 筛子
  • fission 分裂
  • whose 谁的
  • pombe 非洲粟酒
  • activity 活度
  • ablation 剥蚀