Cell-specific non-canonical amino acid labelling identifies changes in the de novo proteome during memory formation
作者: Harrison Tudor EvansLiviu-Gabriel BodeaJürgen Götz
作者单位: 1Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute The University of Queensland Brisbane Australia
刊名: eLife, 2020, Vol.9
来源数据库: eLife Sciences Publications, Ltd
DOI: 10.7554/eLife.52990
关键词: Long-term memoryNon-canonical amino acid labellingProtein synthesisHippocampusAzidonorleucineMRNA splicing
原始语种摘要: The formation of spatial long-term memory (LTM) requires the de novo synthesis of distinct sets of proteins; however, a non-biased examination of the de novo proteome in this process is lacking. Here, we generated a novel mouse strain, which enables cell-type-specific labelling of newly synthesised proteins with non-canonical amino acids (NCAAs) by genetically restricting the expression of the mutant tRNA synthetase, NLL-MetRS, to hippocampal neurons. By combining this labelling technique with an accelerated version of the active place avoidance task and bio-orthogonal non-canonical amino acid tagging (BONCAT) followed by SWATH quantitative mass spectrometry, we identified 156 proteins that were altered in synthesis in hippocampal neurons during spatial memory formation. In addition to...
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  • formation 建造
  • canonical 标准的
  • amino 氨基
  • labelling 贴标签
  • memory 记忆
  • proteins 蛋白质
  • synthesis 合成
  • specific 
  • distinct 相异的
  • splicing 接合