Widefield imaging of upconverting nanoparticles on epifluorescence microscopes adapted for laser illumination with top-hat profile
作者: Jiri MrazekMartina PospisilovaVit SvozilOndrej CadekKristina NesporovaRomana SulakovaMartina BrandejsovaJana VranovaVladimir Velebny
作者单位: 1Charles University in Prague
2Contipro Biotech
刊名: Journal of Biomedical Optics, 2016, Vol.21 (5), pp.056007-056007
来源数据库: SPIE-the International Society for Optical Engineering
DOI: 10.1117/1.JBO.21.5.056007
关键词: upconverting nanoparticlesbioimagingfluorescence microscopylaser beam homogenization
原始语种摘要: We describe a modification of epifluorescence microscopes that allows quantitative widefield imaging of samples labeled by upconverting nanoparticles (UCNP). A top-hat illumination profile on the sample was achieved with a 980-nm laser diode by using tandem microlens arrays, a moving diffuser and a telescope, which adjusts the top-hat area to the field of view. Illumination homogeneity is a critical factor for imaging of UCNP since the intensity of their luminescence typically scales with the second power of the excitation intensity. Our illuminator is combined with the epifluorescence attachment of the microscope, allowing easy switching between observation of UCNP and traditional fluorescent dyes. Illumination profile homogeneity of about 98% was measured for objectives with...
全文获取路径: SPIE  (合作)
影响因子:2.881 (2012)

  • illumination 光照强度
  • profile 纵断图
  • imaging 图像形成
  • homogeneity 均匀性
  • illuminator 照明
  • capability 能力
  • demonstrate 说明
  • moving 移动
  • efficient 有用的
  • laser 激光