Structured polarized light microscopy for collagen fiber structure and orientation quantification in thick ocular tissues
作者: Bin YangBryn BrazileNing-Jiun JanYi HuaJunchao WeiIan A. Sigal
作者单位: 1University of Pittsburgh School of Medicine
2University of Pittsburgh
刊名: Journal of Biomedical Optics, 2018, Vol.23 (10), pp.106001-106001
来源数据库: SPIE-the International Society for Optical Engineering
DOI: 10.1117/1.JBO.23.10.106001
关键词: Polarized light microscopyStructured light illuminationCollagen fiber structureCollagen fiber orientationBiomechanicsEyeSclera
原始语种摘要: Collagen is a major constituent of the eye and understanding its architecture and biomechanics is critical to preserve and restore vision. We, recently, demonstrated polarized light microscopy (PLM) as a powerful technique for measuring properties of the collagen fibers of the eye, such as spatial distribution and orientation. Our implementation of PLM, however, required sectioning the tissues for imaging using transmitted light. This is problematic because it limits analysis to thin sections. This is not only slow, but precludes study of dynamic events such as pressure-induced deformations, which are central to the role of collagen. We introduce structured polarized light microscopy (SPLM), an imaging technique that combines structured light illumination with PLM to allow imaging and...
全文获取路径: SPIE  (合作)
影响因子:2.881 (2012)

  • orientation 定向
  • microscopy 显微镜检查
  • light 
  • illumination 光照强度
  • collagen 胶骨硬蛋白质
  • fiber 纤维
  • visualization 目测
  • polarized 极化的
  • thick 厚的
  • demonstrate 说明