Clinical lymphocytes construction for light scattering inversion study: a three-dimensional morphology constructed method from defective confocal images
作者: Lu ZhangYunhao XieYingzhe TuLele LuoKaixing LiLi YuanWei ChenHong ZhaoZhenxi Zhang
作者单位: 1Xi’an Jiaotong University
刊名: Journal of Biomedical Optics, 2018, Vol.23 (8), pp.085003-085003
来源数据库: SPIE-the International Society for Optical Engineering
DOI: 10.1117/1.JBO.23.8.085003
关键词: Label freeScatteringMorphologyLymphocyte
原始语种摘要: Constructing models of cells’ realistic internal and external morphology is vital for correlation between light scattering and morphology of the scattering structure. The image stack obtained from fluorescent confocal microscopy is at present used to construct the cell’s three-dimensional (3-D) morphology. However, due to the poor labeling quality and unavoidable optical noise present in the image stacks, 3-D morphologies are difficult to construct and are an impediment to the statistical analyses of cell structures. We propose a method called the “area and shape constraint method (ASCM)” for constructing 3-D morphology. Blurred 3-D morphologies constructed by common methods from image stacks considered as defective and which are commonly discarded are well restored by the ASCM....
全文获取路径: SPIE  (合作)
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影响因子:2.881 (2012)

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关键词翻译
关键词翻译
  • morphology 形态学
  • scattering 散射
  • light 
  • inversion 倒转
  • stacks 堆集室
  • confocal 共焦
  • image 
  • method 方法
  • dimensional 量纲的
  • defective 有缺陷的