Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy
作者: Hsun-Chia HsuLei LiJunjie YaoTerence T. W. WongJunhui ShiRuimin ChenQifa ZhouLihong V. Wang
作者单位: 1Washington University in St. Louis
2California Institute of Technology
3University of Southern California
刊名: Journal of Biomedical Optics, 2018, Vol.23 (7), pp.076001-076001
来源数据库: SPIE-the International Society for Optical Engineering
DOI: 10.1117/1.JBO.23.7.076001
关键词: Optical-resolution photoacoustic microscopyLimited view phenomenonOblique illuminationMouse cerebral diving vessels
原始语种摘要: Optical-resolution photoacoustic microscopy (OR-PAM) has demonstrated fast, label-free volumetric imaging of optical-absorption contrast within the quasiballistic regime of photon scattering. However, the limited numerical aperture of the ultrasonic transducer restricts the detectability of the photoacoustic waves, thus resulting in incomplete reconstructed features. To tackle the limited-view problem, we added an oblique illumination beam to the original coaxial optical-acoustic scheme to provide a complementary detection view. The virtual augmentation of the detection view was validated through numerical simulations and tissue-phantom experiments. More importantly, the combination of top and oblique illumination successfully imaged a mouse brain in vivo down to 1 mm in depth, showing...
全文获取路径: SPIE 
影响因子:2.881 (2012)

  • illumination 光照强度
  • microscopy 显微镜检查
  • optical 光学的
  • photoacoustic 光声的
  • reconstructed 修]重构[建
  • phantom 受精台
  • detectability 探测率
  • incomplete 末完成的
  • detection 探测
  • resolution 分解能力