High-throughput ultraviolet photoacoustic microscopy with multifocal excitation
作者: Toru ImaiJunhui ShiTerence T. W. WongLei LiLiren ZhuLihong V. Wang
作者单位: 1California Institute of Technology
2Washington University in St. Louis
刊名: Journal of Biomedical Optics, 2018, Vol.23 (3), pp.036007-036007
来源数据库: SPIE-the International Society for Optical Engineering
DOI: 10.1117/1.JBO.23.3.036007
关键词: Photoacoustic imagingUltraviolet laserSurgical margin assessmentMicrolens array1-D array ultrasonic transducer
原始语种摘要: Ultraviolet photoacoustic microscopy (UV-PAM) is a promising intraoperative tool for surgical margin assessment (SMA), one that can provide label-free histology-like images with high resolution. In this study, using a microlens array and a one-dimensional (1-D) array ultrasonic transducer, we developed a high-throughput multifocal UV-PAM (MF-UV-PAM). Our new system achieved a 1.6 ± 0.2 μm lateral resolution and produced images 40 times faster than the previously developed point-by-point scanning UV-PAM. MF-UV-PAM provided a readily comprehensible photoacoustic image of a mouse brain slice with specific absorption contrast in ∼16 min, highlighting cell nuclei. Individual cell nuclei could be clearly resolved, showing its practical potential for intraoperative SMA.
全文获取路径: SPIE  (合作)
影响因子:2.881 (2012)

  • photoacoustic 光声的
  • transducer 转换器
  • throughput 吞吐量
  • microscopy 显微镜检查
  • array 阵列
  • multifocal 多病贴的
  • ultrasonic 超音波的
  • image 
  • highlighting 突出性
  • scanning 扫描