Virtual unfolding of light sheet fluorescence microscopy dataset for quantitative analysis of the mouse intestine
作者: Alessia CandeoIlenia SanaEleonora FerrariLuigi MaiuriCosimo D’AndreaGianluca ValentiniAndrea Bassi
作者单位: 1Politecnico di Milano
2European Institute for Research in Cystic Fibrosis
3University of Piemonte Orientale
刊名: Journal of Biomedical Optics, 2016, Vol.21 (5), pp.056001-056001
来源数据库: SPIE-the International Society for Optical Engineering
DOI: 10.1117/1.JBO.21.5.056001
关键词: three-dimensional microscopylight sheet fluorescence microscopyimage processing
原始语种摘要: Light sheet fluorescence microscopy has proven to be a powerful tool to image fixed and chemically cleared samples, providing in depth and high resolution reconstructions of intact mouse organs. We applied light sheet microscopy to image the mouse intestine. We found that large portions of the sample can be readily visualized, assessing the organ status and highlighting the presence of regions with impaired morphology. Yet, three-dimensional (3-D) sectioning of the intestine leads to a large dataset that produces unnecessary storage and processing overload. We developed a routine that extracts the relevant information from a large image stack and provides quantitative analysis of the intestine morphology. This result was achieved by a three step procedure consisting of: (1) virtually...
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影响因子:2.881 (2012)

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关键词翻译
关键词翻译
  • intestine 
  • mouse 小鼠
  • microscopy 显微镜检查
  • quantitative 量的
  • fluorescence 荧光
  • analysis 分析
  • sheet 岩席
  • statistically 统计地
  • intestinal 肠的
  • light