Delayed-onset of procoagulant signalling revealed by kinetic analysis of COAT platelet formation
作者: Lorenzo AlberioCatherine RavanatB&eacuteatrice HechlerPierre H. ManginFran&ccedilois LanzaChristian Gachet
作者单位: 1Lorenzo Alberio, MD, Service et Laboratoire central d’hématologie, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon 46, 1011 Lausanne, Switzerland, Tel.: +41 21 314 34 32, Fax: +41 21 314 43 23, E-mail: lorenzo.alberio@chuv.ch
刊名: Thromb Haemost, 2017, Vol.117 (6), pp.1101-1114
来源数据库: Schattauer GmbH
DOI: 10.1160/TH16-09-0711
关键词: PlateletsprocoagulantCOATsurfaceintracellular signallingcalciumphosphorylationapoptosisflow cytometry
原始语种摘要: The combined action of collagen and thrombin induces the formation of COAT platelets, which are characterised by a coat of procoagulant and adhesive molecules on their surface. Although recent work has started to highlight their clinical relevance, the exact mechanisms regulating the formation of procoagulant COAT platelets remain unclear. Therefore, we employed flow cytometry in order to visualise in real time surface and intracellular events following simultaneous platelet activation with convulxin and thrombin. After a rapid initial response pattern characterised by the homogenous activation of the fibrinogen receptor glycoprotein IIb/IIIa in all platelets, starting with a delay of about 2 minutes an increasing fraction transforms to procoagulant COAT platelets. Their surface is...
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影响因子:6.094 (2012)

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关键词翻译
关键词翻译
  • formation 建造
  • signalling 发信号
  • procoagulant 促凝血的
  • observed 观察到的
  • dissect 分析
  • distinct 相异的
  • maintain 
  • expression 表示
  • dichotomous 二歧分枝的
  • recent 近来的