In vitro induction of the acrosome reaction in spermatozoa from endangered Spanish bulls: Effect of breed, culture media and incubation time
作者: S. Demyda-PeyrasJ. DoradoM. HidalgoL. De LucaA. Muñoz-SerranoM. Moreno-Millan
作者单位: 1Faculty of Agricultural Sciences, Lomas de Zamora University, 1836 Buenos Aires, Argentina
2Animal Reproduction Group, Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Cordoba, 14071 Cordoba, Spain
3Department of Genetics, Faculty of Veterinary Medicine, University of Cordoba, 14071 Cordoba, Spain
刊名: Livestock Science, 2012, Vol.149 (3), pp.275-281
来源数据库: Elsevier Journal
DOI: 10.1016/j.livsci.2012.07.025
关键词: Cattle breedAcrosome reactionCryopreserved spermFluorescent techniques
原始语种摘要: Abstract(#br)Current conservation programs for endangered species have been widely benefited from the use of modern assisted reproductive techniques. However, at the present time, there is little information available regarding the use of locally adapted cattle breeds on IVF programs, and even less about their sperm behavior. The aims of this study were to (1) evaluate standard acrosome reaction (AR) protocols in cryopreserved semen doses of Andalusian endangered cattle breeds, and (2) investigate the effect of breed, culture medium supplementation and incubation time on AR test results. To this end, 80 frozen semen doses from 16 bulls (5 semen samples per bull) of five different cattle breeds (3 Berrendo en Colorado, 6 Berrendo en Negro, 2 Cardena Andaluza, 1 Pajuna and 4 Retinta) were...
全文获取路径: Elsevier  (合作)
影响因子:1.249 (2012)