Zona-free and with-zona parthenogenetic embryo production in goat ( Capra hircus ) — effect of activation methods, culture systems and culture media
作者: Arun Kumar DeDhruba MalakarManoj Kumar JenaRahul DuttaShweta GargYogesh S. Akshey
作者单位: 1Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
刊名: Livestock Science, 2011, Vol.143 (1), pp.35-42
来源数据库: Elsevier Journal
DOI: 10.1016/j.livsci.2011.08.012
关键词: ParthenogenesisGoatNuclear transferBlastocyst
原始语种摘要: Abstract(#br)Studies on parthenogenetic activation of oocytes are important to improve the efficiency of nuclear transfer as artificial activation of oocytes is an essential component of nuclear transfer protocol. The present study was carried out to investigate the effects of different activation methods, culture systems and culture media on in vitro development of zona-free and with-zona parthenogenetic embryos in goat. In case of zona-free parthenogenesis, there was a significant (p<0.05) increase in cleavage rate and blastocyst yield when oocytes were activated by electrical pulse (76.29±0.52% and 19.07±0.39% respectively) than when Ca-ionophore was used for activation...
全文获取路径: Elsevier  (合作)
影响因子:1.249 (2012)