Adjuvant and protective properties of native and recombinant Bordetella pertussis adenylate cyclase toxin preparations in mice
| 作者： | Kalantar Hormozi, Roger Parton, John Coote |
| 作者单位： |
1Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Glasgow University, Joseph Black Building, Glasgow G12 8QQ, UK |
| 刊名： | FEMS Immunology and Medical Microbiology, 1999, Vol.23 (4), pp.273-282 |
| 来源数据库： | Elsevier Journal |
| DOI： | 10.1016/S0928-8244(98)00146-1 |
| 关键词： | Adenylate cyclase toxin; Bordetella pertussis; Adjuvant; |
| 原始语种摘要： | Abstract(#br)Bordetella pertussis produces a cell-invasive adenylate cyclase toxin which is synthesised from the cyaA gene as an inactive protoxin that is post-translationally activated by the product of the cyaC gene. Purified active and inactive CyaA proteins were prepared from B. pertussis or from recombinant Escherichia coli expressing both cyaA and cyaC genes or the cyaA gene alone, respectively. In addition, a hybrid toxin (Hyb2) in which an internal region of CyaA had been replaced with the analogous region from the leukotoxin (LktA) of Pasteurella haemolytica , and which had low cell-invasive activity, was also prepared from E. coli expressing the cyaC gene. The CyaA preparations showed no evidence of toxicity in a mouse weight-gain test. Active toxin preparations were protective... |
