Predicted molecular structure of the mammalian cell entry protein Mce1A of Mycobacterium tuberculosis
作者: Amit Kumar DasDevrani MitraMorten HarboeBidisha NandiRobin E. HarknessDebabrata DasHarald G. Wiker
作者单位: 1Department of Biotechnology, Indian Institute of Technology, Kharagpur 721 302, West Bengal, India
2Institute of Immunology, Rikshospitalet University Hospital, NO-0027 Oslo, Norway
3Aventis Pasteur, Connaught Campus, Toronto, Ont., Canada M2 3T4
4National Veterinary Institute, Oslo, Norway
5Department of Environmental Immunology, Norwegian Institute of Public Health, NO-0403 Oslo, Norway
刊名: Biochemical and Biophysical Research Communications, 2003, Vol.302 (3), pp.442-447
来源数据库: Elsevier Journal
DOI: 10.1016/S0006-291X(03)00116-5
关键词: Mammalian cell entry proteinMolecular modelMycobacterium tuberculosisMonoclonal antibodyEpitope
原始语种摘要: Abstract(#br)The proposed role of the mammalian cell entry protein 1A (Mce1A) of Mycobacterium tuberculosis is to facilitate invasion of host cells. The structure of Mce1A was modelled on the basis of the crystal structure of Colicin N of Escherichia coli by fold prediction and threading. Mce1A, as the model predicts, is an α/β protein consisting of two major (α and β) domains, connected by a long α helix. The model further revealed that the protein contains 12 helices, 9 strands, and 1 turn. The final model of Mce1A was verified through the program VERIFY 3D and more than 90% of the residues were in the favourable region. A mouse monoclonal antibody, TB1–5 76C, is directed to an epitope within a 60-mer peptide that has been shown to promote uptake of bacteria in mammalian cells. We show...
全文获取路径: Elsevier  (合作)