Simultaneous Measurement of Desaturase Activities Using Stable Isotope Tracers or a Nontracer Method
作者: Hui-Min SuJ.Thomas Brenna
作者单位: 1Division of Nutritional Sciences, Cornell University, Ithaca, New York, 14853
刊名: Analytical Biochemistry, 1998, Vol.261 (1), pp.43-50
来源数据库: Elsevier Journal
DOI: 10.1006/abio.1998.2706
原始语种摘要: Abstract(#br)We report here methods for simultaneous determination of in vitro desaturase activities using stable isotope tracers or using a nontracer method in place of radiotracers. Rat microsomal Δ 6 - and Δ 9 -desaturase activities were assayed using standard incubationmedium by monitoring the increase in product dueto the reactions [U- 13 C]18:2 n -6 → [U- 13 C]18:3 n -6 or [U- 13 C]16:0 → [U- 13 C]16:1 n -7, respectively. The stable isotope measurements were made by high-precision gas chromatography–combustion isotope ratio mass spectrometry. Reaction-dependent changes in product or substrate concentrations were also monitored quantitatively by conventional capillary gas chromatography with flame-ionization detection. Desaturase activities calculated from these data are consistent...
全文获取路径: Elsevier  (合作)
影响因子:2.582 (2012)