Chitosan coated carbon fiber microelectrode for selective in vivo detection of neurotransmitters in live zebrafish embryos
作者: Rıfat Emrah ÖzelKenneth N. WallaceSilvana Andreescu
作者单位: 1Department of Chemistry and Biomolecular Science, 8 Clarkson Ave, Potsdam, NY 136995810, USA
2Department of Biology, Clarkson University, Potsdam, NY 136995810, USA
刊名: Analytica Chimica Acta, 2011, Vol.695 (1), pp.89-95
来源数据库: Elsevier Journal
DOI: 10.1016/j.aca.2011.03.057
关键词: Carbon fiber microelectrodeChitosanAscorbic acidInterferencesIn vivo detection of neurotransmittersZebrafishIntestine
原始语种摘要: Abstract(#br)We report the development of a chitosan modified carbon fiber microelectrode for in vivo detection of serotonin. We find that chitosan has the ability to reject physiological levels of ascorbic acid interferences and facilitate selective and sensitive detection of in vivo levels of serotonin, a common catecholamine neurotransmitter. Presence of chitosan on the microelectrode surface was investigated using scanning electron microscopy (SEM) and cyclic voltammetry (CV). The electrode was characterized using differential pulse voltammetry (DPV). A detection limit of 1.6nM serotonin with a sensitivity of 5.12nA/μM, a linear range from 2 to 100nM and a reproducibility of 6.5% for n =6...
全文获取路径: Elsevier  (合作)
影响因子:4.387 (2012)