Interaction between mammalian cells and Pasteurella multocida B:2. Adherence, invasion and intracellular survival
作者: Sarah OthmanRoger PartonJohn Coote
作者单位: 1Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, B215, Level 2, Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow G12 8TA, United Kingdom
刊名: Microbial Pathogenesis, 2012, Vol.52 (6), pp.353-358
来源数据库: Elsevier Journal
DOI: 10.1016/j.micpath.2012.03.005
关键词: Pasteurella multocidaBacterial invasionEmbryonic bovine lung cells
英文摘要: Abstract(#br)A Pasteurella multocida B:2 strain from a case of bovine haemorrhagic septicaemia (HS) and a derivative, JRMT12, that was attenuated by a deletion in the aroA gene, were shown to adhere to, invade and survive within cultured embryonic bovine lung (EBL) cells. By comparison, bovine strains of Mannheimia haemolytica serotype A1 and P. multocida serotype A:3, although able to adhere to EBL cells, were not found intracellularly. The B:2 strains were viable intracellularly over a 7 h period, although a steady decline in viability was noted with time. Entry into the mammalian cells was inhibited by cytochalasin D, indicating that cell uptake was by an actin-dependent process. Viability assessment of EBL cells by trypan blue staining indicated that none of the bacterial strains was...
全文获取路径: Elsevier  (合作)
影响因子:1.974 (2012)