Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements
作者: Trang Thi Phuong PhanHoang Duc NguyenWolfgang Schumann
作者单位: 1Institute of Genetics, University of Bayreuth, D-95445 Bayreuth, Germany
2Center for Bioscience and Biotechnology, University of Science, Vietnam National University-Ho Chi Minh City, 227 Nguyen Van Cu, District 5, Ho Chi Minh City, Viet Nam
3Laboratory of Molecular Biotechnology, University of Science, Vietnam National University-Ho Chi Minh City, 227 Nguyen Van Cu, District 5, Ho Chi Minh City, Viet Nam
刊名: Journal of Biotechnology, 2012, Vol.157 (1), pp.167-172
来源数据库: Elsevier Journal
DOI: 10.1016/j.jbiotec.2011.10.006
关键词: UP element−35 and −10 elementsITPG-induction
原始语种摘要: Abstract(#br)Transcription efficiency of inducible promoters remains a bottleneck in recombinant protein production in Bacillus subtilis cells. Here, we present experimental data how to generate strong IPTG-inducible promoters by optimization of nucleotides at the conserved regions of the groESL promoter including the UP element, the −35, −15, −10 and the +1 region. Combination of these changes into one promoter enhanced the amount of recombinant proteins accumulating intracellularly up to about 30% of the total cellular protein.
全文获取路径: Elsevier  (合作)
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影响因子:3.183 (2012)

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