Use of single-layer centrifugation with Androcoll-C to enhance sperm quality in frozen-thawed dog semen
作者: J. DoradoM.J. GálvezJ.M. MorrellL. AlcarázM. Hidalgo
作者单位: 1Animal Reproduction Group, Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Cordoba, Córdoba, Spain
2Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, Uppsala, Sweden
刊名: Theriogenology, 2013, Vol.80 (8), pp.955-962
来源数据库: Elsevier Journal
DOI: 10.1016/j.theriogenology.2013.07.027
关键词: Dog spermatozoaSperm separationSperm qualityMotility patternsCryopreservation
英文摘要: Abstract(#br)The aim of this study was to investigate whether single-layer centrifugation (SLC) with Androcoll-C could select good quality spermatozoa, including those with specific motility patterns, from doses of frozen dog semen. Semen from five dogs was collected and cryopreserved following a standard protocol. After thawing, the semen samples were divided in two aliquots, one of which was used as a control and the other one processed by SLC. Assessment of sperm motility (assessed by computer-assisted semen analysis), morphology (Diff-Quick staining), viability (dual staining with propidium iodine/acridine orange), and acrosome integrity (dual staining with propidium iodine/isothiocyanate-labeled peanut [ Arachis hypogaea ] agglutinin) were performed on aliquots of fresh semen,...
全文获取路径: Elsevier  (合作)
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影响因子:2.082 (2012)

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