DNA integrity of canine spermatozoa during chill storage assessed by the sperm chromatin dispersion test using bright-field or fluorescence microscopy
作者: M. HidalgoM. UrbanoI. OrtizS. Demyda-PeyrasM.R. MurabitoM.J. GálvezJ. Dorado
作者单位: 1Veterinary Reproduction Group, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Córdoba, Córdoba, Spain
2MERAGEM Research Group, Department of Genetics, Faculty of Veterinary Medicine, University of Cordoba, Cordoba, Spain
刊名: Theriogenology, 2015, Vol.84 (3), pp.399-406
来源数据库: Elsevier Journal
DOI: 10.1016/j.theriogenology.2015.03.030
关键词: Sperm DNASperm chromatin dispersionHalomaxFluorescenceBright field
英文摘要: Abstract(#br)The objective of this study was to evaluate the effect of chill storage on canine sperm DNA fragmentation assessed by the sperm chromatin dispersion test using bright-field microscopy with Wright solution (sDF-B) or fluorescence microscopy with propidium iodide (sDF-F). The relationship and agreement between the results obtained with both staining methods were analyzed. The values of DNA fragmentation indexes (sDF-F and sDF-B) were compared at each time of chill storage (0, 24, 48, 72, and 96 hours). Additionally, the sperm DNA fragmentation rate (slope) was compared between the methods during chill storage. Good agreement and no significant differences between values obtained with both staining procedures were observed. Finally, the effect of chill storage for up to 96 hours...
全文获取路径: Elsevier  (合作)
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影响因子:2.082 (2012)

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