Construction of a 5′-controllable stabilizing element (CoSE) for over-production of heterologous proteins at high levels in Bacillus subtilis
作者: Trang Thi Phuong PhanHoang Duc NguyenWolfgang Schumann
作者单位: 1Institute of Genetics, University of Bayreuth, Universitaetsstr. 30, D-95445 Bayreuth, Germany
2University of Science, Vietnam National University Ho Chi Minh City, 227 Nguyen Van Cu, District 5, Ho Chi Minh, Viet Nam
刊名: Journal of Biotechnology, 2013, Vol.168 (1), pp.32-39
来源数据库: Elsevier Journal
DOI: 10.1016/j.jbiotec.2013.07.031
关键词: RBSCoSEBacillus subtilismRNA stabilityOverexpression
原始语种摘要: Abstract(#br)Different mRNA stabilizing elements including the 3′-stem-loop, the ribosome binding sites (RBS), the 5′-stem-loop and the spacer region between the RBS and the 5′-stem-loop were analysed in detail to increase mRNA stability resulting in enhanced expression of heterologous proteins. In addition, in combination with mRNA stabilizing elements, we propose a new class of 5′-mRNA controllable stabilizing element (CoSE) which is composed of a transcriptional operator such as lacO of the Escherichia coli lac operon and a suitable RBS followed by an optimal spacer length. Such a CoSE allowed Bacillus subtilis cells to synthesize extraordinary stable transcripts with a half-life of the model bgaB reporter transcript (codes for an β-galactosidase gene derived from Bacillus...
全文获取路径: Elsevier  (合作)
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影响因子:3.183 (2012)

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