Genetic and serological surveillance for non-primate hepacivirus in horses in Japan
作者: Aya MatsuuSeiji HoboKunihide AndoTakashi SanekataFumio SatoYoshiro EndoTomohiko AmayaTomohiro OsakiMasayuki HorieTatsunori MasataniMakoto OzawaKyoko Tsukiyama-Kohara
作者单位: 1Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary, Kagoshima University, Korimoto, Kagoshima, Japan
2Laboratory of Domestic Animal Internal Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan
3Bloodhorse Training Center Horse Clinic, Urakawa, Hokkaido, Japan
4International Animal Health and Management College, Setagaya, Tokyo, Japan
5Department of Equine Breeding Science, Hidaka Training and Research Center, Japan Racing Association, Urakawa, Hokkaido, Japan
6Miyazaki Yearling Training Farm, Japan Racing Association, Hanagashima, Miyazaki, Japan
7Yamatokohgen Animal Medical Clinic, Habikino, Osaka, Japan
8Laboratory of Veterinary Surgery, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan
9Laboratory of Animal Hygiene, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan
刊名: Veterinary Microbiology, 2015, Vol.179 (3-4), pp.219-227
来源数据库: Elsevier Journal
DOI: 10.1016/j.vetmic.2015.05.028
关键词: Non-primate hepacivirusHorseGaussia luciferase immunoprecipitation system
原始语种摘要: Abstract(#br)Non-primate hepacivirus (NPHV) is a recently discovered homolog of the hepatitis C virus in horses. The frequency and distribution of NPHV infections among horses in Japan is unknown. In this study, serum samples from 453 horses across Japan were screened for NPHV RNA using real-time RT-PCR and anti-nonstructural 3 protein (NS3) antibodies using the Gaussia luciferase immunoprecipitation system assay. In order to monitor the course of NPHV infection in horses, we examined 31 stored samples (9 adult horses and 22 young horses) obtained one year ago and compared the results to the recent data. Stored sera from 7 mare–foal pairs were also examined. The NS3 region sequences of 14 NPHV strains from NPHV RNA positive serum samples were determined and analyzed phylogenically. Of the...
全文获取路径: Elsevier  (合作)
影响因子:3.127 (2012)