Validated high performance thin layer chromatography method for simultaneous determination of quercetin and gallic acid in Leea indica
作者: Ankita A. PatelAeshna A. AminArpit H. PatwariMamta B. Shah
作者单位: 1Department of Pharmacognosy, L. M. College of Pharmacy, Ahmedabad, Gujarat, India
刊名: Revista Brasileira de Farmacognosia, 2017, Vol.27 (1), pp.50-53
来源数据库: Elsevier Journal
DOI: 10.1016/j.bjp.2016.05.017
关键词: Gallic acidHPTLCLeea indicaQuercetinSimultaneous estimationValidation
原始语种摘要: Abstract(#br)A sensitive and reliable high performance thin layer chromatography method has been developed for the simultaneous estimation of quercetin and gallic acid in Leea indica , Vitaceae. Ethyl acetate extract prepared from hydrolysed aqueous alcoholic extract (70%) was applied on silica gel G 60 F 254 plate. The plate was developed using toluene-ethyl acetate-formic acid, 5:4:1 (v/v/v) as a mobile phase and detection and quantification were performed by densitometric scanning at 254nm. The system was found to give well resolved bands for quercetin ( R f 0.63) and gallic acid ( R f 0.45) from other constituents present in the extract of L. indica . The correlation coefficient was found to be 0.991 and 0.999 with relative standard deviation, 0.97–1.23% and...
全文获取路径: Elsevier  (合作)
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