Molecular characterization of two kiss genes and their expression in rohu ( Labeo rohita ) during annual reproductive cycle
作者: Ashis SahaAvinash PradhanSushmita SenguptaMadhusmita NayakMrinal SamantaLakshman SahooShiba Shankar Giri
作者单位: 1Division of Fish Nutrition and Physiology, ICAR—Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India
2Division of Fish Health Management, ICAR—Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India
3Division of Fish Genetics and Biotechnology, ICAR—Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, India
刊名: Comparative Biochemistry and Physiology, Part B, 2016, Vol.191 , pp.135-145
来源数据库: Elsevier Journal
DOI: 10.1016/j.cbpb.2015.10.008
关键词: kiss1kiss2RohuBrainPituitaryGonadAnnual reproductive cycle
原始语种摘要: Abstract(#br)Kisspeptin is an important regulator of reproduction in mammals and presumably non-mammalian species. In the Indian subcontinent, rohu ( Labeo rohita ) is a commercially important seasonal breeder freshwater fish species, but till date, the expression of kisspeptin gene during different phases of annual reproductive cycle has not been investigated. To address this, we cloned and characterized kiss1 and kiss2 full-length cDNA by RACE (rapid amplification of cDNA ends), and analyzed their expressions in brain–pituitary–gonad (BPG) axis by quantitative real time PCR (qRT-PCR) assay at various gonadal developmental stages of the annual reproductive cycle. Full-length rohu kiss1 and kiss2 cDNA encodes 116 and 125 amino acids respectively, and in the adult fish, they were widely...
全文获取路径: Elsevier  (合作)
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