Electrogenic steps of light-driven proton transport in ESR, a retinal protein from E xiguobacterium sibiricum
作者: Sergey A. SiletskyMahir D. MamedovEvgeniy P. LukashevSergei P. BalashovDmitriy A. DolgikhAndrei B. RubinMikhail P. KirpichnikovLada E. Petrovskaya
作者单位: 1Belozersky Institute of Physical-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russian Federation
2Department of Biology, Lomonosov Moscow State University, 119234 Moscow, Leninskie gory, 1, Russian Federation
3Department of Physiology and Biophysics, University of California, Irvine 92697, USA
4Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Ul. Miklukho-Maklaya, 16/10, Russian Federation
刊名: BBA - Bioenergetics, 2016, Vol.1857 (11), pp.1741-1750
来源数据库: Elsevier Journal
DOI: 10.1016/j.bbabio.2016.08.004
关键词: Retinal proteinLight energy transductionProton pumpPhotocyclePhotoelectric potential generationCharge transfer steps
英文摘要: Abstract(#br)A retinal protein from Exiguobacterium sibiricum (ESR) functions as a light-driven proton pump. Unlike other proton pumps, it contains Lys96 instead of a usual carboxylic residue in the internal proton donor site. Nevertheless, the reprotonation of the Schiff base occurs fast, indicating that Lys96 facilitates proton transfer from the bulk. In this study we examined kinetics of light-induced transmembrane electrical potential difference, ΔΨ, generated in proteoliposomes reconstituted with ESR. We show that total magnitude of ΔΨ is comparable to that produced by bacteriorhodopsin but its kinetic components and their pH dependence are substantially different. The results are in agreement with the earlier finding that proton uptake precedes reprotonation of the Schiff base in...
全文获取路径: Elsevier  (合作)