Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for the identification of Neisseria gonorrhoeae
作者: R. BuchananD. BallH. DolphinJ. Dave
作者单位: 1Division of Infection, Barts Health NHS Trust, London, UK
2Public Health England, London, UK
刊名: Clinical Microbiology and Infection, 2016, Vol.22 (9), pp.815.e5-815.e7
来源数据库: Elsevier Journal
DOI: 10.1016/j.cmi.2016.06.010
关键词: API NHMatrix-assisted laser desorption-ionization time-of-flight mass spectrometryNeisseria gonorrhoeaeSexually transmitted infectionPathogen identification
英文摘要: Abstract(#br)Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) was compared with the API NH biochemical method for the identification of Neisseria gonorrhoeae in routine clinical samples. A retrospective review of laboratory records for 1090 isolates for which both biochemical and MALDI-TOF MS identifications were available was performed. Cases of discrepant results were examined in detail for evidence supportive of a particular organism identification. Of 1090 isolates, 1082 were identified as N. gonorrhoeae by API NH. MALDI-TOF MS successfully identified 984 (91%) of these after one analysis, rising to 1081 (99.9%) after two analyses, with a positive predictive value of 99.3%. For those isolates requiring a repeat analysis, failure to generate...
全文获取路径: Elsevier  (合作)
分享到:

×