Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling
作者: Xiaolong LuoHsuan-Chen WuChen-Yu TsaoYi ChengJordan BetzGregory F. PayneGary W. RubloffWilliam E. Bentley
作者单位: 1Institute for Bioscience and Biotechnology Research (IBBR), University of Maryland, College Park, MD 20742, USA
2Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA
3Institute for Systems Research (ISR), University of Maryland, College Park, MD 20742, USA
4Department of Materials Science and Engineering, University of Maryland, College Park, MD 20742, USA
刊名: Biomaterials, 2012, Vol.33 (20), pp.5136-5143
来源数据库: Elsevier Journal
DOI: 10.1016/j.biomaterials.2012.03.037
关键词: BiofabricationBiofilm mimicsCell signalingQuorum sensingChitosan membraneAlginate hydrogel
英文摘要: Abstract(#br)Signaling between cells guides biological phenotype. Communications between individual cells, clusters of cells and populations exist in complex networks that, in sum, guide behavior. There are few experimental approaches that enable high content interrogation of individual and multicellular behaviors at length and time scales commensurate with the signal molecules and cells themselves. Here we present “biofabrication” in microfluidics as one approach that enables in-situ organization of living cells in microenvironments with spatiotemporal control and programmability. We construct bacterial biofilm mimics that offer detailed understanding and subsequent control of population-based quorum sensing (QS) behaviors in a manner decoupled from cell number. Our approach reveals...
全文获取路径: Elsevier  (合作)
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影响因子:7.604 (2012)

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