Rumi Is a CAP10 Domain Glycosyltransferase that Modifies Notch and Is Required for Notch Signaling
作者: Melih AcarHamed Jafar-NejadHideyuki TakeuchiAkhila RajanDafina IbraniNadia A. RanaHongling PanRobert S. HaltiwangerHugo J. Bellen
作者单位: 1Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
2Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
3Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
4Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, Stony Brook University, Stony Brook, NY 11794-5215, USA
刊名: Cell, 2007, Vol.132 (2), pp.247-258
来源数据库: Elsevier Journal
DOI: 10.1016/j.cell.2007.12.016
原始语种摘要: Summary(#br)Notch signaling is broadly used to regulate cell-fate decisions. We have identified a gene, rumi, with a temperature-sensitive Notch phenotype. At 28°C–30°C, rumi clones exhibit a full-blown loss of Notch signaling in all tissues tested. However, at 18°C only a mild Notch phenotype is evident. In vivo analyses reveal that the target of Rumi is the extracellular domain of Notch. Notch accumulates intracellularly and at the cell membrane of rumi cells but fails to be properly cleaved, despite normal binding to Delta. Rumi is an endoplasmic reticulum-retained protein with a highly conserved CAP10 domain. Our studies show that Rumi is a protein O -glucosyltransferase, capable of adding glucose to serine residues in Notch EGF repeats with the consensus C 1 -X-S-X-P-C 2 sequence....
全文获取路径: Elsevier  (合作)
影响因子:31.957 (2012)