Prolonged exposure to arecoline arrested human KB epithelial cell growth: Regulatory mechanisms of cell cycle and apoptosis
作者: Po-Hsuen LeeMei-Chi ChangWen-Hui ChangTong-Mei WangYing-Jen WangLiang-Jiunn HahnYuan-Soon HoChuan-Yu LinJiiang-Huei Jeng
作者单位: 1Laboratory of Dental Pharmacology & Toxicology, Department of Dentistry, National Taiwan University Hospital, National Taiwan University Medical College, No. 1, Chang-Te Street, Taipei 100, Taiwan
2Team of Biomedical Science, Chang-Gung Institute of Technology, Taoyuan, Taiwan
3Department of Applied Chemistry, Chung-Shan Medical University, Taichung, Taiwan
4Department of Environmental Medicine, National Cheng Kung University, Tainan, Taiwan
5School of Biomedical Technology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan
刊名: Toxicology, 2005, Vol.220 (2), pp.81-89
来源数据库: Elsevier Journal
DOI: 10.1016/j.tox.2005.07.026
关键词: Areca nutApoptosisArecolineBetel quidCell cycleEpithelial cellsCarcinogenesis
原始语种摘要: Abstract(#br)Arecoline, the main areca alkaloid in betel quid (BQ), is reported to have cytotoxic, genotoxic, and mutagenic effects in various cells. It shows strong correlation to the incidence of oral submucous fibrosis, leukoplakia, and oral cancer. To clarify the role of arecoline in BQ-induced carcinogenesis, primary human gingival keratinocyes (GK) and human KB epithelial cells were used for studying the molecular mechanisms of arecoline-mediated cell cycle deregulation for comparison. After 24h of exposure, arecoline (0.2–0.8mM) inhibited KB cell growth in a dose- and time-dependent manner with a reduction in cell number by 27–37 and 37–58%, respectively, as determined by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and...
全文获取路径: Elsevier  (合作)
影响因子:4.017 (2012)