Single-step method for β-galactosidase assays in Escherichia coli using a 96-well microplate reader
作者: Jorrit SchaeferGoran JovanovicIoly Kotta-LoizouMartin Buck
作者单位: 1Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK
刊名: Analytical Biochemistry, 2016, Vol.503 , pp.56-57
来源数据库: Elsevier Journal
DOI: 10.1016/j.ab.2016.03.017
关键词: LacZB-Galactosidase (Bgal)β-GalactosidaseMicroplate reader
英文摘要: Abstract(#br)Historically, the lacZ gene is one of the most universally used reporters of gene expression in molecular biology. Its activity can be quantified using an artificial substrate, o -nitrophenyl-ß- d -galactopyranoside (ONPG). However, the traditional method for measuring LacZ activity (first described by J. H. Miller in 1972) can be challenging for a large number of samples, is prone to variability, and involves hazardous compounds for lysis (e.g., chloroform, toluene).(#br)Here we describe a single-step assay using a 96-well microplate reader with a proven alternative cell permeabilization method. This modified protocol reduces handling time by 90%.
全文获取路径: Elsevier  (合作)
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影响因子:2.582 (2012)

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