Evaluation of cytotoxic activity of protein extracts from the leaves of Morinda pubescens on human cancer cell lines
作者: Anvy Susan ThomasRupachandra SaravanakumarPratiksha V. Gupta
作者单位: 1Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Kancheepuram, Tamil Nadu, India
刊名: Revista Brasileira de Farmacognosia, 2017, Vol.27 (1), pp.99-104
来源数据库: Elsevier Journal
DOI: 10.1016/j.bjp.2016.08.003
关键词: Cytotoxic activityFTIR analysisMorinda pubescensMTT assayProtein extraction
原始语种摘要: Abstract(#br)Biologically active proteins isolated from plant species can be used in traditional medicine as prolific resources for new drugs Morinda pubescens Sm., Rubiaceae, is a promising medicinal plant which is widely used in folk medicine to treat fever due to primary complex, ulcer and glandular swellings. In this study, proteins were extracted from the leaves of M. pubescens , and precipitated with ammonium sulphate at various saturation concentrations ranging from 20 to 80%. The precipitated protein sample obtained with 80% saturation was further purified using ultrafiltration membrane (<10kDa). SDS-PAGE analysis identified the presence of crude and ultrafiltered protein bands. FTIR spectrum of the ultrafiltered protein fractions depicted the presence of...
全文获取路径: Elsevier  (合作)