Simultaneous Determination of Methionine and Homocysteine by on-column derivatization with o -phtaldialdehyde
作者: Kamila BorowczykGrażyna ChwatkoPaweł KubalczykHieronim JakubowskiJolanta KubalskaRafał Głowacki
作者单位: 1University of Łódź, Faculty of Chemistry, Department of Environmental Chemistry, 163 Pomorska Str., 90-236 Łódź, Poland
2Department of Biochemistry and Biotechnology, University of Life Sciences, 11 Dojazd Str., 60-632 Poznań, Poland
3Institute of Bioorganic Chemistry, 12/14 Noskowskiego Str., 61-704 Poznań, Poland
4Department of Microbiology, Biochemistry & Molecular Genetics, Rutgers University, New Jersey Medical School, International Center for Public Health, Newark, NJ 07103-3535, USA
5Department of Metabolic Diseases, Endocrinology and Diabetology, Children's Memorial Health Institute, Warszawa, Poland
刊名: Talanta, 2016, Vol.161 , pp.917-924
来源数据库: Elsevier Journal
DOI: 10.1016/j.talanta.2016.09.039
关键词: HyperhomocysteinemiaOn-column derivatizationHigh performance liquid chromatographyBiological fluids
原始语种摘要: Abstract(#br)A fast and simple HPLC-based assay has been developed for the simultaneous determination of homocysteine (Hcy) and methionine (Met) in plasma and urine samples, utilizing as small volume of sample as 10μL. The assay uses on-column derivatization with o -phthaldialdehyde. The separation of Hcy and Met was achieved in 14min on a reversed phase C-18 column, followed by fluorescence detection (excitation at 348nm and emission at 438nm for Met; excitation at 370nm and emission at 480nm for Hcy). Linearity of the detector response was observed in the range of 2–60 μmol L −1 for Met and 2–40 μmol L −1 for Hcy. The method was...
全文获取路径: Elsevier  (合作)
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影响因子:3.498 (2012)

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