Rapid and sensitive diagnosis of fungal keratitis with direct PCR without template DNA extraction
作者: G. ZhaoH. ZhaiQ. YuanS. SunT. LiuL. Xie
作者单位: 1Shandong Provincial Key Laboratory of Ophthalmology, Shandong Provincial Excellent Innovation Team Programme, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China
刊名: Clinical Microbiology and Infection, 2014, Vol.20 (10), pp.O776-O782
来源数据库: Elsevier Journal
DOI: 10.1111/1469-0691.12571
关键词: Diagnosisdirect PCRfungal keratitisrapidsensitive
原始语种摘要: Abstract(#br)This study was aimed at developing a direct PCR assay without template DNA extraction for the rapid and sensitive diagnosis of infectious keratitis. Eighty corneal scrapings from 67 consecutive patients with clinically suspected infectious keratitis were analysed prospectively. Direct PCR was performed with all scrapings, with specific primers for fungi, bacteria, herpes simplex virus-1 (HSV-1) and Acanthamoeba simultaneously. The results were compared with those obtained from culture, smear, and confocal microscopy. Discrepant results were resolved according to the therapeutic effects of the corresponding antimicrobial drugs. The lowest detection limit of direct PCR was ten copies of each pathogen. Sixty-six scrapings yielded positive results with direct PCR, giving a total...
全文获取路径: Elsevier  (合作)
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