Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: revolutionizing clinical laboratory diagnosis of mould infections
作者: M. GautierS. RanqueA.-C. NormandP. BeckerA. PackeuC. CassagneC. L'OllivierM. HendrickxR. Piarroux
作者单位: 1Parasitology & Mycology, Assistance Publique-Hôpitaux de Marseille, CHU Timone-Adultes, Marseilles, France
2Aix-Marseille University, UMR MD3 IP-TPT, Marseilles, France
3Mycology and Aerobiology Section, BCCM/IHEM: Scientific Institute of Public Health, Brussels, Belgium
刊名: Clinical Microbiology and Infection, 2014, Vol.20 (12), pp.1366-1371
来源数据库: Elsevier Journal
DOI: 10.1111/1469-0691.12750
关键词: Fungihumansmassmatrix-assisted laser desorption ionization time-of-flightmicrobiological techniquesmycosesspectrometry
原始语种摘要: Abstract(#br)The clinical diagnosis of mould infections currently involves complex species identification based on morphological criteria, which is often prone to error. Employing an extensive mould species reference spectral library (up to 2832 reference spectra, corresponding to 708 strains from 347 species), we assessed the extent to which matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) enhanced the accuracy of species identification. MALDI-TOF MS data were validated against morphology-based and DNA sequence-based results with 262 clinical isolates collected over a 4-month period in 2013. The implementation of MALDI-TOF MS resulted in a dramatic improvement in mould identification at the species level (from 78.2% to 98.1%) and a marked...
全文获取路径: Elsevier  (合作)