Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis
作者: Trang Thi Phuong PhanHoang Duc NguyenWolfgang Schumann
作者单位: 1Institute of Genetics, University of Bayreuth, D-95440 Bayreuth, Germany
刊名: Protein Expression and Purification, 2005, Vol.46 (2), pp.189-195
来源数据库: Elsevier Journal
DOI: 10.1016/j.pep.2005.07.005
关键词: IPTGgroE promoterlac operator
英文摘要: Abstract(#br)Two plasmid-based expression vectors have been constructed where one allows intracellular production of recombinant proteins while the second directs the proteins into the culture medium. Both vectors use the strong promoter preceding the groESL operon (codes for the essential heat shock proteins GroES and GroEL) of Bacillus subtilis fused to the lac operator allowing their induction by addition of ITPG. While the background level of expression of these expression cassettes is very low in the absence of the inducer, an induction factor of about 1300 was measured. When the genes htpG and pbpE (coding for a heat shock protein and a penicillin-binding protein, respectively) were fused to the groE promoter, the amount of recombinant protein produced after addition of IPTG...
全文获取路径: Elsevier  (合作)
影响因子:1.429 (2012)