Efficient gene modulation in mouse epiblast using a Sox2Cre transgenic mouse strain
作者: Shigemi HayashiPaula LewisLarysa PevnyAndrew P. McMahon
作者单位: 1Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
刊名: Mechanisms of Development, 2002, Vol.119 , pp.S97-S101
来源数据库: Elsevier Journal
DOI: 10.1016/S0925-4773(03)00099-6
关键词: Sox2CreRecombinaseloxPMox2 ( MORE )Sonic hedgehogR26RZ/APEpiblastVisceral endodermExtraembryonic tissue
原始语种摘要: Abstract(#br)We have generated a transgenic line that expresses the Cre gene product under the regulation of a 12.5 kb upstream regulatory sequence from the Sox2 gene. Using a R26R reporter line, we show that this transgenic line induces recombination in all epiblast cells by embryonic day (E) 6.5 but little or no activity in other extraembryonic cell types at this time. When crossed to a conditional allele of the Sonic hedgehog gene ( Shh c ), all Sox2Cre;Shh n /Shh c embryos displayed a phenotype indistinguishable from that of the Shh null mutant. Sox2Cre functioned more efficiently in epiblast-mediated recombination than the Mox2Cre ( MORE ) transgenic line, which has also been shown to drive Cre -mediated recombination exclusively in the embryonic component of the early mouse embryo....
全文获取路径: Elsevier  (合作)
影响因子:2.383 (2012)