Generation of a prostate epithelial cell-specific Cre transgenic mouse model for tissue-specific gene ablation
作者: Xiantuo WuJian WuJiapeng HuangWilliam C. PowellJianFeng ZhangRobert J. MatusikFrank O. SangiorgiRobert E. MaxsonHenry M. SucovPradip Roy-Burman
作者单位: 1Department of Pathology, Keck School of Medicine, University of Southern California, 2011 Zonal Avenue, Los Angeles, CA 90089, USA
2Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, 2011 Zonal Avenue, Los Angeles, CA 90089, USA
3Department of Urologic Surgery, Vanderbilt University School of Medicine, A-1302 Medical Center North, Nashville, TN 37232, USA
4Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, 2011 Zonal Avenue, Los Angeles, CA 90089, USA
刊名: Mechanisms of Development, 2001, Vol.101 (1), pp.61-69
来源数据库: Elsevier Journal
DOI: 10.1016/S0925-4773(00)00551-7
关键词: Bacteriophage DNA recombinaseProstate-specific gene knockoutRXRα gene deletionProstatic epithelial-specific gene targetingTransgenic miceR26R lacZ allele
原始语种摘要: Abstract(#br)To facilitate the elucidation of the genetic events that may play an important role in the development or tumorigenesis of the prostate gland, we have generated a transgenic mouse line with prostate-specific expression of Cre recombinase. This line, named PB-Cre4, carries the Cre gene under the control of a composite promoter, ARR 2 PB which is a derivative of the rat prostate-specific probasin (PB) promoter. Based on RT-PCR detection of Cre mRNA in PB-Cre4 mice or Cre-mediated activation of LacZ activity in PB-Cre4/R26R double transgenic mice, it is conclusively demonstrated that Cre expression is post-natal and prostatic epithelium-specific. Although the Cre recombination is detected in all lobes of the mouse prostate, there is a significant difference in expression levels...
全文获取路径: Elsevier  (合作)
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影响因子:2.383 (2012)

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