Properties of the basal calcium influx in human red blood cells
作者: Roman HudecBoris LakatošKarin KaiserováJozef OrlickýL'udovı́t Varečka
作者单位: 1Department of Biochemistry and Microbiology, Slovak University of Technology, Radlinského 9, Bratislava 812 37, Slovak Republic
2Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, 833 34 Bratislava, Slovak Republic
刊名: BBA - Biomembranes, 2004, Vol.1661 (2), pp.204-211
来源数据库: Elsevier Journal
DOI: 10.1016/j.bbamem.2004.01.011
关键词: Red blood cellBasal Ca 2+ influxUncoupler
原始语种摘要: Abstract(#br)The basal 45 Ca 2+ influx in human red blood cells (RBC) into intact RBC was measured. 45 Ca 2+ was equilibrated with cells with t 1/2 =15–20 s and the influx reached the steady state value in about 90–100 s and the steady state level was 1.5±0.2 μmol/l packed cells ( n =6) at 37 °C. The average value of the Ca 2+ influx rate was 43.2±8.9 μmol/l packed cells hour. The rate of the basal influx was pH-dependent with a pH optimum at pH 7.0 and on the temperature with the temperature optimum at 25 °C. The basal Ca 2+ influx was saturable with Ca 2+ up to 5 mmol/l but at higher extracellular Ca 2+ concentrations caused further increase of basal Ca 2+ influx. The 45 Ca 2+ influx was stimulated by addition of submicromolar concentrations of phorbol esters (phorbol...
全文获取路径: Elsevier  (合作)