Development of a carbohydrate binding assay for the B-oligomer of pertussis toxin and toxoid
作者: Sheena R. GomezDorothy K-L. XingMichael J. CorbelJohn CooteRoger PartonChun-Ting Yuen
作者单位: 1Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
2Laboratory for Molecular Structure, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
3Division of Infection and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK
刊名: Analytical Biochemistry, 2006, Vol.356 (2), pp.244-253
来源数据库: Elsevier Journal
DOI: 10.1016/j.ab.2006.05.012
关键词: Pertussis toxinToxoidCarbohydrate binding assayOligosaccharidesGlycoproteins
英文摘要: Abstract(#br)Pertussis toxin (PTx) is a major virulence factor produced by Bordetella pertussis and, in its detoxified form PTd, is an important component of pertussis vaccines. The in vivo histamine sensitization test (HIST) is currently used for the safety testing of these vaccines. However, an alternative test is needed because of large assay variability and ethical concerns with regard to animal usage. PTx has two functionally distinct domains: the enzymatic A-protomer and the B-oligomer that facilitates host-cell binding and entry of PTx into the cell. The development of a quantitative PTx binding assay using glycoproteins or defined oligosaccharides is reported. PTx was found to bind preferentially to multiantennary N -glycans, with the highest binding toward the fully sialylated...
全文获取路径: Elsevier  (合作)
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影响因子:2.582 (2012)

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