Protein coated microcrystals formulated with model antigens and modified with calcium phosphate exhibit enhanced phagocytosis and immunogenicity
作者: Sarah JonesCatpagavalli AsokanathanDorota KmiecJune IrvineRoland FleckDorothy XingBarry MooreRoger PartonJohn Coote
作者单位: 1Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, University Avenue, Glasgow G12 8QQ, UK
2Division of Bacteriology, National Institute of Biological Standards and Control (NIBSC), Blanche Lane, South Mimms, Hertfordshire EN6 3QG, UK
3Division of Cellular Biology and Imaging, National Institute of Biological Standards and Control (NIBSC), Blanche Lane, South Mimms, Hertfordshire EN6 3QG, UK
4Department of P&A Chemistry, WestChem, Thomas Graham Building, 295 Cathedral Street, Glasgow G1 1XL, UK
5XstalBio Ltd., CIDS, Thomson Building, University Avenue, Glasgow G12 8QQ, UK
刊名: Vaccine, 2014, Vol.32 (33), pp.4234-4242
来源数据库: Elsevier Journal
DOI: 10.1016/j.vaccine.2013.09.061
关键词: MicroparticlesCalcium phosphatePhagocytosisAdjuvant
原始语种摘要: Abstract(#br)Protein-coated microcrystals (PCMCs) were investigated as potential vaccine formulations for a range of model antigens. Presentation of antigens as PCMCs increased the antigen-specific IgG responses for all antigens tested, compared to soluble antigens. When compared to conventional aluminium-adjuvanted formulations, PCMCs modified with calcium phosphate (CaP) showed enhanced antigen-specific IgG responses and a decreased antigen-specific IgG1:IgG2a ratio, indicating the induction of a more balanced Th1/Th2 response. The rate of antigen release from CaP PCMCs, in vitro , decreased strongly with increasing CaP loading but their immunogenicity in vivo was not significantly different, suggesting the adjuvanticity was not due to a depot effect. Notably, it was found that CaP...
全文获取路径: Elsevier  (合作)
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影响因子:3.492 (2012)

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