Selection and validation of endogenous controls for microRNA expression studies in endometrioid endometrial cancer tissues
作者: Anna TorresKamil TorresPaulina WdowiakTomasz PaszkowskiRyszard Maciejewski
作者单位: 1Laboratory of Biostructure, Chair and Department of Human Anatomy, Medical University of Lublin, Jaczewskiego 4, 20-090, Lublin, Poland
2General and Oncologic Surgery Department, Lublin County Specialist Hospital, 20-718, Al. Krasnicka 100, Lublin, Poland
3III Chair and Department of Gynecology, Medical University of Lublin, Jaczewskiego 8, Lublin, Poland
刊名: Gynecologic Oncology, 2013, Vol.130 (3), pp.588-594
来源数据库: Elsevier Journal
DOI: 10.1016/j.ygyno.2013.06.026
关键词: MicroRNAEndometrioid endometrial cancerReference geneEndogenous controlReal-time qPCR
原始语种摘要: Abstract(#br)Objectives(#br)microRNAs comprise a family of small, non-coding RNAs, which regulate gene expression at the posttranscriptional level. Multiple studies implicated important roles of microRNAs in various malignancies including endometrioid endometrial carcinoma (EEC). qPCR is widely used in the studies investigating microRNA expression. Relative quantification of microRNA expression requires proper normalization methods and endogenous controls are widely used for this purpose. The aim of this study was experimental identification of stable endogenous controls for normalization of microRNA qPCR expression studies in EEC.(#br)Methods(#br)Expression of twelve candidate endogenous controls (miR-16, miR-26b, miR-92a, RNU44, RNU48, U75, U54, U6, U49, RNU6B, RNU38B, U18A) was...
全文获取路径: Elsevier  (合作)
影响因子:3.929 (2012)

  • endometrial 子宫内膜的
  • endometrioid 子宫内膜样的
  • endogenous 内成的
  • controls 控制机构
  • expression 表示
  • normalization 正规化
  • endometrium 子宫内膜
  • candidate 选择物
  • cancer 癌症
  • regulate 控制