A comparative study on the interactions of human copper chaperone Cox17 with anticancer organoruthenium(II) complexes and cisplatin by mass spectrometry
作者: Lijie LiWei GuoKui WuXuelei WuLinhong ZhaoYao ZhaoQun LuoYuanyuan WangYangzhong LiuQingwu ZhangFuyi Wang
作者单位: 1Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, National Centre for Mass Spectrometry in Beijing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China
2School of Chemical and Environmental Engineering, China University of Mining and Technology, Beijing 100083, PR China
3Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, PR China
4University of Chinese Academy of Sciences, Beijing 100049, PR China
刊名: Journal of Inorganic Biochemistry, 2016, Vol.161 , pp.99-106
来源数据库: Elsevier Journal
DOI: 10.1016/j.jinorgbio.2016.05.008
关键词: Organoruthenium complexesCisplatinCox17Anticancer agentsLC-ESI-MS
英文摘要: Abstract(#br)Herein we report investigation of the interactions between anticancer organoruthenium complexes, [(η 6 -arene)Ru(en)(Cl)]PF 6 (en=ethylenediamine, arene= p -cymene ( 1 ) or biphenyl ( 2 )), and the human copper chaperone protein Cox17 by mass spectrometry with cisplatin as a reference. The electrospray ionization mass spectrometry (ESI-MS) results indicate much weaker binding of the ruthenium complexes than that of cisplatin to apo-Cox17 2s-s , the functional state of Cox17. Up to tetra-platinated Cox17 adducts were identified while only mono-ruthenated and a little amount of di-ruthenated Cox17 adducts were detected even for the reactions with 10-fold excess of the Ru complexes. However, ESI-MS...
全文获取路径: Elsevier  (合作)
影响因子:3.197 (2012)