Gold nanoparticles based molecular beacons for in vitro and in vivo detection of the matriptase expression on tumor
作者: Dawei DengDongyin ZhangYang LiSamuel AchilefuYueqing Gu
作者单位: 1Department of Biomedical Engineering, China Pharmaceutical University, Nanjing 210009, PR China
2Department of Radiology, School of Medicine, Washington University, St. Louis, MO 63110, USA
刊名: Biosensors and Bioelectronics, 2013, Vol.49 , pp.216-221
来源数据库: Elsevier Journal
DOI: 10.1016/j.bios.2013.05.018
关键词: Molecular beaconGold nanoparticleMatriptasePeptide substrateIn vitro and in vivo detection
原始语种摘要: Abstract(#br)Matriptase, a type II transmembrane serine protease, is responsible for the early stage proliferation of a wide range of human cancers. It is highly expressed on the surface of tumor cells, allowing the protease to serve as a biomarker of cancer detection. Hence, in this study, we designed two molecular beacons consisting of a fluorescent dye–peptide conjugate attached to gold nanoparticles (Au NPs) for in vitro and in vivo detection of the matriptase expression on tumor cells. The peptide substrate (GRQSRAGC) for matriptase served as the linker between dye (donor) and Au NP (acceptor). In this configuration, the dye fluorescence was quenched by Au NP under physiological conditions and recovered after selective cleavage of the GRQSRAGC by matriptase. To maximize spectral...
全文获取路径: Elsevier  (合作)
影响因子:5.437 (2012)