Identification and the preliminary in vitro characterization of IRIS homologue from salivary glands of Ixodes persulcatus Schulze
作者: Kochi ToyomaneSatoru KonnaiAyano NiwaNaftaly GithakaMasayoshi IsezakiShinji YamadaTakuya ItoAi TakanoShuji AndoHiroki KawabataShiro MurataKazuhiko Ohashi
作者单位: 1Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
2Hokkaido Institute of Public Health, Sapporo, Japan
3Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan
4National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo, Japan
刊名: Ticks and Tick-borne Diseases, 2016, Vol.7 (1), pp.119-125
来源数据库: Elsevier Journal
DOI: 10.1016/j.ttbdis.2015.09.006
关键词: Ixodes persulcatusIpisImmunosuppressantIFN-γCell proliferation
原始语种摘要: Abstract(#br)Ixodes ricinus immunosuppressor (Iris) is a tick salivary gland protein derived from I. ricinus . In this study, Iris homolog was identified in the salivary glands of Ixodes persulcatus , which is the specific vector of the Lyme disease agent in Japan. The homolog was named Ipis-1. To investigate the function of Ipis-1, we prepared a recombinant Ipis-1 expressed in COS-7 cells as a rabbit IgG Fc-fused protein (Ipis-1-Ig). Cell proliferation assay and IFN-γ ELISA showed that Ipis-1-Ig inhibits the proliferation and IFN-γ production of bovine peripheral blood mononuclear cells (PBMCs). Notably, Ipis-1-Ig inhibited the cell proliferation and production of IFN-γ in bovine PBMCs even when CD14 + cells were depleted, suggesting that Ipis could directly interact with T cells and...
全文获取路径: Elsevier  (合作)
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影响因子:2.353 (2012)

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